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百螢課堂之花青染料Cy3酰肼的化學(xué)光譜特性

2023-08-23 13:04 作者:百螢生物  | 我要投稿

1.百螢 Cy3酰肼概述

Cy3 酰肼是美國AAT Bioquest生產(chǎn)的Cy系列染料,花菁染料Cy3,常被應(yīng)用于生物分子標(biāo)記,熒光成像及其他熒光生物分析。他們廣泛應(yīng)用于多肽,蛋白,核苷酸等。Cy3染料與蛋白質(zhì)結(jié)合后熒光大幅度增強。

圖1.Cy3酰肼的化學(xué)結(jié)構(gòu)


2.百螢 Cy3酰肼參數(shù)? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Ex(nm)? ? ? ? ?555

Em(nm) ???????569

分 子 量? ? ? ??872.85

溶? ?? ?劑? ? ? ??DMSO

存儲條件? ? ? ?在-15℃以下保存,避光防潮

?

3.?百螢 Cy3酰肼化學(xué)光譜特性

校正因子(260nm)? ? ??0.07

校正因子(280nm)? ? ?0.073

Ex(nm)? ? ? ? ? ? ? ? ? ? ? 555

Em(nm)? ? ? ? ? ? ? ? ?? ?569

圖2.Cy3酰肼化學(xué)光譜特性

4.百螢 Cy3酰肼參考文獻

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors:?Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal:?Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors:?Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal:?Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors:?Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal:?Journal of Biomaterials Applications (2017): 923--932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors:?Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal:?Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors:?Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal:?ACS applied materials & interfaces (2016): 17068--17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors:?John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal:?Nat Chem Biol (2016): 459--465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors:?Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal:?Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors:?Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal:?Journal of Biological Chemistry (2016): 10426--10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors:?Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal:?Journal of Chromatography B (2015): 187--193

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors:?Lu Xiao, Jia Guo
Journal:?Analytical Methods (2015): 7290--7295


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